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KMID : 0043320010240050407
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Archives of Pharmacal Research
2001 Volume.24 No. 5 p.407 ~ p.411
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Quantitative Determination of Eleutheroside B and I from Acanthopanax Species by High Performance Liquid Chromatography
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Kang Jong-Seong
Linh Pham-Tuan
Cai Xing-Fu
Kim Hang-Sup
Lee Jung-Joon
Kim Young-Ho
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Abstract
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Reversed-phase high performance liquid chromatographic method was applied for the determination of eleutheroside B and E in the various Acanthopanax species collected In Korea. The stationary phase used was Zorbax 300 SB and a mobile phase program was used, which started at 6% acetonitrile for 2 min, and then a linear gradient was operated for the next 18 min to 17% acetonitrile at a flow rate of 1.0 ml/min. The column effluent was monitored at UV 210 nm. Identification was carried out by comparing the retention time and the LC/MS spectrum of each peak corresponding to eleutheroside B and E from sample with those of standards. In general, the contents of eleutheroside B and 1 in stems were higher than those In roots. Acanthopanax species could be classified into two groups based upon the contents of eleutheroside B and E: one group contains no or very little eleutheroside B and another contains both eleutheroside B and E.
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KEYWORD
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Acanthopanax species, Eleutheroside B, Eleutheroside E, Gradient elution, HPLC
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